State of Utah Center of Excellence for Biomedical Microfluidics
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| Students involved: JungKyu Kim and Richard Eich A novel method for patterning a nanoporous aluminum oxide membrane is being developed using SU-8 to allow for extraction of DNA from multiple samples simultaneously. DNA extraction was performed on the patterned membrane using multiple wells to show the ability to collect multiple DNA samples simultaneously as shown in the figure. DNA, marked with a fluorescent dye, was collected on the membrane and successfully observed using a fluorescence microscope. The uniformity of the extraction process was measured. The techniques developed in this work have application to lab-on-a-chip type systems that include DNA extraction steps.
Figure 1. DNA extraction on the patterned
membrane. A,C,D) After extracting
the DNA on the patterned membrane, B) Before extracting the DNA on the patterned
membrane. JungKyu Kim, Karl Voelkerding, and Bruce K. Gale, "Patterning of an nanoporous membrane for multi-sample DNA extraction," Journal of Micromechanics and Microengineering, Vol. 16, pp. 33-39, 2006. |
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