State of Utah Center of Excellence for Biomedical Microfluidics
|
|
| Students involved: Ryan Sincic Micropatterning Multiple Cell Lines for Parallel Screening of Chemical Agents Our current project involves developing a technology for creating microarrays of cells in a 96 well tissue culture plate. This technique localizes cells into islands on the surface of a cell culture treated substrate. These microspots of cells contain ~2000cells/mm2 (see figure 1). Each spot may contain a different cell type enabling the parallel screening of multiple cell types with the same chemical environment. The process utilizes a micro-stenciling tool with a microfluidic handling platform. This technique uniquely does not rely on a hydrogel matrix for substrate attachment making the system more comparable to conventional cell culture approaches. The current testing involves screening the drug makaluvamine H with an array of chinese hamster ovarian cell lines (AA8-WT, XRS-6, UV20, EM9). Makaluvamines are topoisomerase inhibiters and are particularly toxic to cell lines deficient in repair of DNA double strand breaks. The current experiments are conducted to take data on the cell line specific toxicity of makaluvamine H.
figure 1. Cells localized into a 200x400 um island on a collagen substrate. |
Send mail to
bruce.gale@utah.edu with
questions or comments about this web site.
|
